Sometimes these things take time, especially if you are changing what you are doing.

What is the issue with her room being untidy? Is it a big problem for you? I was wondering whether leaving it might be a good idea? I bet, as soon as she's having problems finding a favourite toy, or has no space to play, she might be more inclined to listen - then you can say 'well, perhaps we can tidy it together?'. What do you think? I know it's annoying when she's being disrespectful but a lot of her pain is coming out. Punishment is only going to make it worse and her harder to reach.