Oh dear mattjstead
I'm sorry to hear about the difficult situation you've been put in now that your wife is on holiday and you're getting interrupting comments. I think you've been doing your best juggling the demands of looking after your kids and keeping the business afloat.
The only thing I can think to suggest is maybe you have a conversation, timing is important, about what you and her want whilst she is on holiday. Otherwise perhaps she will continue to place these demands on you without realising how anxious and already stressed you are? And the two of you can reach some kind of consensus, where both parties are happy with how the rest of the holiday whilst your wife is at home will go.
You deserve to have peace of mind.