My limited experience with Diflucan involved 1 prescription, 2 pills only and it worked very quickly. Your doc may have a different protocol. But that is the beauty of Diflucan - if it's going to work, it's going to work in 1 or 2 doses. If I were you, I would be on the phone 1st thing in the morning getting another appt , hopefully by the end of the day. Especially if you are not seeing any relief by the time you wake up (and not masked by any antihistamines you might be taking to cope with the itch.) Be pushy if you have to (not rude, but assertive.) I would insist on a a culture, too. But, that's just me. That said, maybe the Rx will really kick in by morning.