Yes, Seroquel has caused me twitching. It didn't start to do that until my dose went up to at least 400 mg, and especially once I got to 600 mg. My hands involuntarily twitch and when I am sitting, I feel my thigh muscles twitch. It isn't noticeable to others, but it's annoying. Sometimes it is difficult to keep my hand steady. Luckily, it's not that frequent...but my pdoc suggested either going down on the dose a little bit or taking another medication for the shaking. I went down to 400 mg and cannot go any lower than that or I'm afraid I'll go manic, but unfortunately, there isn't that much of a difference with the twitching at 400 mg for me (so far....this is a recent change though). I'll probably just try to deal with it, but if it gets more frequent, I'll ask my pdoc about the medicine she might recommend.