Roll Call 20

i wonder where punky is.

I'm really sorry that you have been feeling down. I'm glad you did post because I was worried about you How did your appointment go? I hope you will be able to find a support group.

What happened with your experiment at work today sometimesp?

Me too!!!! I miss her and am worried about her. I wish I could just drive over to her house and check on her.

lol thats a long drive. if you did i mean.
she hasnt been on in a few days. or a couple or so.

Ha, thanks ! I'm normally real quiet when I firsts meet people. After I get use to people I'm more open to people.

Right! I checked her page and left her a private message. No response from her so far

Something tells me she's busy with visiting her daughter in the hospital and stuff like that. Hope she comes back soon. I miss her.

holy crap... I'm already above 1400 post? It took me forever to get to 1000. I've must been really chatty with you guys then. lol

i was under my 1200 calorie goal. sometimesp said 1200 is healthy way to lose it so i messed up today and ate more than 500. but kept right under 1200. i have a weight loss diary im keeping and my goal everyday is 1200 or under. but i really wannna keep it around 500. im drinking lots of water and tea and some coffee.

i really want to buy an espresso maker. because its a lot of money to get espresso at starbucks (which is the only place i know of to go to plus i know the people there and theyre real nice to me anyways. the guy actually gave me a free shot of espresso). hes a real cute guy close to my age. has tattoos and medium blond hair. SO CUTE.

Oh it was bizarre but at least part of it worked so I got to go home early. So I'm using a newer cell type to measure something called TER...we kind of zap the cells and look at electrical resistance of the monolayer of cells this tells us how easily ions can move past the sides of the cells and this relates to water movement and thus diarrhea. Anyway I was testing two lines of controls with our bacterial infection. All the data I have so far suggests that expressing extra copies of a certain magic protein protects these gates on the side of the cell that are called tight junctions which should in theory prevent diarrhea...well what I did today suggests that even though the gates are structurally intact they are wide open letting all the ions pass freely just like they weren't there at all. These supposedly protective cells are actually worse than a normal cell. So the data is entirely opposite what I expected this is only the first experiment though so we have to do it a couple of times more to ensure its a real pattern.

Anyway the reason I thought it would take so long is the bacteria normally cause a decrease in TER by 4 hours but the last time I tried this experiment it didn't decrease even by 6 hours so I read about this cell line and some people follow it out 10 hours but it takes another 4 hours to grow and prep the bacteria. Turns out all I needed was a higher dose of bacteria and they responded well but the cells also had to be at least two weeks old. Anyway by about 2 hours post infection I was already seeing a decrease so I knew that even though the experiment was not what I expected (hint it never is...seriously) I still got to go home before 9pm so that was a big plus

Stick around I'm at 2566 and haven't even been here a year

That sounds awesome sometimesp! Even if it wasnt what you were aiming for.

A cute boy you say?

Tee hee... go for it!

its a long shot for me. i dont dress nice and dont have nice hair. but i told him i liked his tattoos. and asked him some questions. hes real nice.

jcpenney - Capresso® Mini Espresso Maker - jcpenney

Check this one out...relatively cheap but great reviews....glad you're flirting with the coffee guy

Yeah it's often more fun when something unexpected happens...

thanks for this! i might buy it when i get some more money. i so wish i could take a pic with him and show you guys. hes real cute. especially in his little starbucks uniform.

Wow, that's something. My goal is to get premier Poohbah because apparently there's a forum open to those who have that status.

yea there is.

im almost to 10,000 posts. it wont be long.

i would have more posts but there was one time where i didnt come here for 6 months.

Ooh a secret club well now I'm sticking around for sure! At this rate about 2.5 months and I'll be there.

lol I know what you mean. I don't dress nicely at all and my hair get's greasy after a day. Talking to my ex boyfriend one day while at a convention he suggested I should dress nicer and maybe that'll make me feel better about myself. Which mean's I have to ditch my sweat pants and get back into jeans. Had a "friend" once make fun of the way I dress at school.. she called me a hobo homo. That was before she found out I was bi. Ha. Then again she was also that friend that said "big boobs don't count when you're fat." I ended up stop being friends with her. She eventually took me off Facebook.

It say's that you're already there!

i see 9200 posts?...